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On the basis of BLAST and phylogenetic results, strain UN01 was identified as L. fermentum UN01. The phylogenetic data described above were obtained by using MEGA4 package using neighbour-joining, minimum evolution,maximum parsimony and bootstrapping methods. A direct analysis of the genetic distance and the phylogenetic treeOn the basis of BLAST and phylogenetic results, strain UN01 was identified as L. fermentum UN01. The phylogenetic data described above were obtained by using MEGA4 package using neighbour-joining, minimum evolution,maximum parsimony and bootstrapping methods. A direct analysis of the genetic distance and the phylogenetic tree (Figure 3) was determined by the 16S rRNA sequence. Various strains have been examined for the phylogenetic relationships. The evolutionary history was inferred using the UPGMA method. The optimal tree for UN01 with the sum of branch length was found to be 0.15751446. In general, 16S rRNA is most frequently used for finding out the phylogenetic relationships between closely related species. From this, the closely related UN01 suggests an evolutionary pathway for the novel form.
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Antibiotic sensitivity of the isolate against viable antibioticsGrowth of isolate UN01 was not inhibited by Co-trimoxacole and Ofloxacin. Growth was sensitive to Erythromycin, Rifampicin and resistance to Streptomycin, Ciproflaxacin, Vancomycin, Chloramphenicol, Ampicillin and Amoxycillin.Antibiotic sensitivity of the isolate against viable antibiotics Growth of isolate UN01 was not inhibited by Co-trimoxacole and Ofloxacin. Growth was sensitive to Erythromycin, Rifampicin and resistance to Streptomycin, Ciproflaxacin, Vancomycin, Chloramphenicol, Ampicillin and Amoxycillin.